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NucleoBond® Xtra Midi EF / Maxi EF
特点：
节约68 % 时间,增加100 % 的产量
- 新一代阴离子交换技术制备超快，高产量，无内毒素的质粒DNA
- 质粒DNA 中内毒素少于0.05 EU/μg DNA，适合对内毒素高敏感的细胞转染
- 专利技术附加的洗涤步骤以去除内毒素-无需额外的冰上放置
- 优化设计的柱子：制备时间约为30 分钟(Midiprep)；35 分钟(Maxi prep)
- 包含柱过滤器：高过虑流速，清除裂解液中的不溶物的同时可向柱子加样
- 改进的硅材料：标准的产量约为250μg(Midi)和1000μg(Maxi)
- NucleoBond®Xtra Plus EF kits 包含NucleoBond®Finalizers可加速 DNA 沉淀
产品概览：
技术 阴离子交换色谱法
产品形式 Midigravity-flow columns Maxigravity-flow columns
裂解液澄清 Columnfilters Columnfilters
样品材料 < 200 mL (high copy) < 600 mL (high copy)
< 400 mL (low copy) < 1200 mL (low copy)
载体大小 < 300 kbp < 300 kbp
产量 250 μg 1000μg
A260/A280 1.80 – 1.95 1.80– 1.95
内毒素水平 < 0.05 EU/μg DNA <0.05 EU/μg DNA
异丙醇 Xtra Midi EF/Xtra Midi Plus EF Xtra Maxi EF/ Xtra Maxi Plus EF
沉淀 Centrifugation/NucleoBond®Finalizer
制备时间 100min / 4 preps 45 min / 4 preps 105 min /4 preps 50 min / 4 preps
应用：
从大肠杆菌中提取高拷贝/低拷贝质粒
订购信息
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产品
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规格
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备注
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货号
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NucleoBond® Xtra Midi EF kits
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NucleoBond® Xtra Midi EF
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10
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NucleoBond® Xtra Midi Columns with inserted NucleoBond® Xtra
Column Filters, buffers, RNase A
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740420.10
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50
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同上
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740420.50
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NucleoBond® XtraMidi Plus EF
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10
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NucleoBond® Xtra Midi Columns with inserted
NucleoBond® Xtra Column Filters, NucleoBond® Finalizer,buffers,
RNase A
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740422.10
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50
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同上
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740422.50
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NucleoBond® Xtra Maxi EF kits
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NucleoBond® Xtra Maxi EF
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10
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NucleoBond® Xtra Maxi Columns with inserted NucleoBond® Xtra
Column Filters, buffers, RNase A
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740424.10
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50
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同上
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740424.50
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NucleoBond® Xtra Maxi Plus EF
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10
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NucleoBond® Xtra Maxi Columns with inserted
NucleoBond® Xtra Column Filters, NucleoBond® Finalizer Large,
buffers, RNase A
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740426.10
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50
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同上
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740426.50
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产品配件
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包装
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备注
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货号
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NucleoBond®Xtra Combi Rack
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for use with NucleoBond® Xtra Midi, Maxi,
AX 100, 500, 2 000, and 10 000 Columns
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740415
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NucleoBond®Xtra EF Buffer Set I
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for isolation of endotoxin-free low-copy plasmids, cosmids,BACs,
PACs, and P1 constructs,only applicable with NucleoBond® Xtra EF
kits,sufficient for 10 Xtra EF Maxi preps or 20 Xtra EF Midi preps,
set includes buffers RES-EF, LYS-EF, NEU-EF, and RNase A
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740427
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